This research is aimed at investigating the effect of spirituality on the employees of Sepah Imam Reza. As this applied research is intended to have a consideration of the effects of deepening spirituality on performance, it has selected an explorative-descriptive method. Furthermore, based on the nature of the topic and research objectives, it has employed survey method as well. Research tools includes two questionnaires of spirituality and staff performance. Data analysis is performed by Pearson correlation coefficient and multiple regression analysis and SPSS software, indicating that employee performance variables affect all components of deepening spirituality, including beliefs, morals and worship, as well as deepening of spirituality itself directly and significantly. This means that employees’ performance at Sepah Imam Reza increases by increasing spirituality and its components. The results also showed that the component of beliefs inserted the most influence on performance. Hence, the research arrived at the conclusion that employees' career belonging and intrinsic motivation can be improved by enhancing spirituality in the workplace, especially by focusing on reinforcing beliefs, and ultimately improve their performance in organizations.